You are here: Products » Antibodies » Primary Antibodies » Stem Cell Antibodies » antibody : Rabbit Anti-Vimentin Polyclonal Antibody

Vimentin Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01193-200	Vimentin Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01193-40	Vimentin Antibody, pAb, Rabbit	40 μg	$50

Full Name

Vimentin Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit anti Vimentin (400-500 aa);

Documents
Document-MSDS: 8678_20081127021811.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
zoom
zoom
zoom
Reference

Description

Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.
GenScript Vimentin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-500 of human vimentin protein (Swiss Prot: P08670).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

Synthetic peptide (KLH-coupled) derived from within residues 400-500 of human vimentin (Swiss Prot: P08670)

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Predicted Band Size

53 kD

Observed Band Size

53 kD, 30 kD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human. This product has not yet been tested with other species.

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.