β-Tubulin III Antibody, pAb, Rabbit

*This product has been discontinued!*

GenScript Rabbit Anti-β-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse β-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone β-tubulin III proteins.

Cat. No.	A01203
Size	Buy in bulk

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Overview Applications Properties Background Bottom Notes

Overview

Specificity	GenScript Rabbit Anti-β-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse β-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone β-tubulin III proteins.
Host Species	Rabbit
Immunogen	Synthetic peptide (KLH-coupled) within residues 150-200 of human β-tubulin III protein (Swiss Prot: Q13509)
Species Reactivity	Human, mouse, and rat. This product has not yet been tested with other species. Reactivity with other β-Tubulin had not been tested yet.
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.5-1 µg/ml
Immunohistochemistry	5-10 µg/ml
Flow cytometry	1-3 µg for 1 x 10⁶ cells
Other applications	User-optimized

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Properties

Form	Lyophilized
Storage Buffer	lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable
Note	Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

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Background

Target Background

Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.GenScript Rabbit Anti Tubulin III Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 150-200 of human Tubulin III protein (Swiss Prot: Q13509).

Synonyms

Rabbit Anti-Beta-Tubulin III pAb;

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Abhay Srivastava, et al. Human induced pluripotent stem cell (hiPSC) line UOMi006-A derived from PBMCs of a patient with Kearns-Sayre syndrome. Stem Cell Res. (2021-04)
2. Leclair NK, et al. Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis. Molecular Cell. (2020-11)
3. Wang M, et al. Functions of Small Organic Compounds that Mimic the HNK-1 Glycan. International Journal of Molecular Sciences. (2020-09)
4. K Hasegawa, et al. Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells. J Cell Biol. (2013-01)