Catalog Products » Antibodies » antibody : Rabbit Anti-β-Catenin Polyclonal Antibody
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A01211-40
40 μg
$79 $47
β-Catenin Antibody, pAb, Rabbitcustom antibody production
β-Catenin Antibody, pAb, Rabbit
Full Name β-Catenin Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti β-Catenin Polyclonal Antibody;
Documents
β-Catenin Antibody, pAb, RabbitDocument-MSDS: 8687_20081127023125.PDF (PDF)
β-Catenin Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
β-Catenin Antibody, pAb, Rabbit zoomβ-Catenin Antibody, pAb, Rabbit
Description
β-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs play a role in mediating the adhesion between cells, signaling the presence of neighboring cells, and anchoring the actin cytoskeleton. In serving these roles, AJs also regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs.
GenScript Rabbit Anti-β-Catenin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 700-750 of human β-Catenin protein (Swiss Prot: P35222).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-β-Catenin Polyclonal Antibody detects endogenous levels of human β-catenin protein. Sequence homology predicts that it will also react with rat β-catenin.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Synthetic peptide (KLH-coupled) within residues 700-750 of human β-Catenin protein (Swiss Prot: P35222)
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Predicted Band Size
85 kD
Observed Band Size
85 kD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human. This product has not yet been tested with other species.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
Citation
Tomas J., et al. Primocolonization is associated with colonic epithelial maturation during conventionalization. FASEB J. 2013 Feb;27(2):645-55.

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