You are here: Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » antibody : Rabbit Anti-Calnexin Polyclonal Antibody

Calnexin Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01234-200	Calnexin Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01234-40	Calnexin Antibody, pAb, Rabbit	40 μg	$50

Full Name

Calnexin Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti Calnexin (N-terminal) Polyclonal Antibody;

Documents
Document-MSDS: 9458_20090225204236.PDF (PDF)
Figures
zoom
zoom
Reference

Description

Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the endoplasmic reticulum (ER) of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.

GenScript Calnexin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide from within N-terminal of human calnexin protein (Swiss Prot: P27824).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Calnexin Antibody, pAb, Rabbit detects endogenous levels of human calnexin protein.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide from within N-terminal of human calnexin (Swiss Prot: P27824)

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

90 kD

Observed Band Size

85 kD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user optimized

Conjugation

Unconjugated

Species Reactivity

Human

Host Species

Rabbit

Antigen Species

Human

Note

Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.