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Calnexin Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01240-200	Calnexin Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01240-40	Calnexin Antibody, pAb, Rabbit	40 μg	$50

Full Name

Calnexin Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-Calnexin pAb;

Documents
Document-MSDS: 9079_20081229202050.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.
GenScript Calnexin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide from carboxyl terminal of human calnexin protein (Swiss Prot: P27824).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Calnexin Antibody, pAb, Rabbit detects endogenous levels of human and mouse calnexin protein. Sequence homology predicts that it will also react with rat, bovine, canine, and Sumatran orangutan calnexin.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

Synthetic peptide (KLH-coupled) from carboxyl terminal of human calnexin (Swiss Prot: P27824).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

90 kD

Observed Band Size

85 kD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human and mouse. This product has not yet been tested with other species.

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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