Catalog Products » Antibodies » Primary Antibodies » Neuroscience Antibodies » Parkin Antibody, pAb, Rabbit
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A01250-40
40 μg
$79 $47
Parkin Antibody, pAb, Rabbitcustom antibody production
Parkin Antibody, pAb, Rabbit
Full Name Parkin Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-Parkin pAb;
Documents
Parkin Antibody, pAb, RabbitDocument-MSDS: 9283_20090121041527.PDF (PDF)
Figures
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Description
Parkin, also known as PARKIN2, is a novel protein in Ubl conjugation pathway. Parkin functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include CCNE1, SYT11, GPR37, and STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. Parkin may play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. The protein may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. Defects in PARK2 are a cause of Parkinson disease (PD).
GenScript Parkin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 300-350 of human parkin protein (Swiss Prot: O60260).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Parkin Antibody, pAb, Rabbit detects endogenous levels of human, mouse and rat parkin protein.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide within residues 300-350 of human parkin protein (Swiss Prot: O60260)
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Predicted Band Size
51 kD
Observed Band Size
51 kD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
IHC: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human, mouse, and rat. This product has not yet been tested with other species.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
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