||Rabbit Anti-Calbindin Polyclonal Antibody;
||Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27 kD protein induced by vitamin D in the duodenums of young birds. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains four active calcium-binding domains and two modified domains that presumably have lost their calcium-binding capacity. The neurons in the brains of patients with Huntington’s disease are calbindin-depleted.
GenScript Rabbit Anti-Calbindin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from residues 150-200 of human calbindin protein (Swiss Prot: P05937).
||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
||GenScript Rabbit Anti-Calbindin Polyclonal Antibody detects human calbindin protein. Sequence homology predicts that it will also to react with mouse and rat calbindin.
||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
||Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
IHC: 5-10 µg/ml
Other applications: user-optimized
Western Blot Protocol(pdf)
Shang Ma., et al. Ric-8A, A Guanine Nucleotide Exchange Factor For Heterotrimeric G Proteins, Regulates Bergmann Glia-Basement Membrane Adhesion During Cerebellar Foliation. J Neurosci. 2012 Oct;32(43):14979 - 14993.