Catalog Products » Antibodies » antibody : Mouse Anti-huFGF-Acidic Monoclonal Antibody
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A01281-40
40 μg
$79 $47
FGF-Acidic Antibody (5F5G8), mAb, Mouse
Full Name FGF-Acidic Antibody (5F5G8), mAb, Mouse
Abbreviated name-1
Mouse Anti-huFGF-Acidic mAb;
Documents
FGF-Acidic Antibody (5F5G8), mAb, MouseDocument-MSDS: 9480_20090304021544.PDF (PDF)
Figures
FGF-Acidic Antibody (5F5G8), mAb, Mouse zoomFGF-Acidic Antibody (5F5G8), mAb, Mouse
Description
FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF-acidic, which is produced in multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist.
Ig Subclass
Mouse IgG1, k
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
This product is specific to human FGF-acidic and shows no cross-reactivity with FGF basic, hFGF6, hFGF16, or hFGF17.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
FGF-acidic full length recombinant protein
Clone ID
5F5G8
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells fused with SP2/0-Ag14 mouse myeloma cells
Conjugation
Unconjugated
Host Species
Mouse
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
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