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A01283-40
40 μg
$79 $47
A01283-200
200 μg(5x40μg)
$275
BMP-7 Antibody (2C2F8), mAb, Mousecustom antibody production
BMP-7 Antibody (2C2F8), mAb, Mouse
Full Name BMP-7 Antibody (2C2F8), mAb, Mouse
Abbreviated name-1
Mouse Anti-huBMP-7 mAb;
Documents
BMP-7 Antibody (2C2F8), mAb, MouseDocument-MSDS: 9492_20090304032227.PDF (PDF)
Figures
Citations
Description
BMP-7, or osteogenic protein 1 (OP-1), is one of at least 15 structurally and functionally related BMPs, all members of the transforming growth factor β (TGF-β) superfamily. BMPs regulate cartilage and bone formation, embryogenesis and morphogenesis of various tissues and organs, and the growth, differentiation, chemotaxis and apoptosis of various cell types. Biologically active BMP-7 is a disulfide-linked homodimer. Cellular responses to BMP-7 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors.
GenScript Human BMP-7 Antibody (2C2F8), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified human recombinant BMP-7 protein (Swiss Prot: P18075).
Ig Subclass
IgG2a, k
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
React with Human BMP-7, not cross-react with BMP-14 protein.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Purified human recombinant BMP-7 protein (Swiss Prot: P18075)
Clone ID
2C2F8
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 0.5-10 µg/ml
ELISA Detection: 0.05-0.2 μg/ml
Recommended antibody pairing for sandwich immunoassay:
Other applications: user-optimized
Fusion Partner
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Conjugation
Unconjugated
Species Reactivity
Human
Host Species
Mouse
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
1
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