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GM-CSF Antibody (4D6C4), mAb, Mouse

Cat. No.	Name	Size	Price
A01290-200	GM-CSF Antibody (4D6C4), mAb, Mouse	200 μg(5x40μg)	$225
A01290-40	GM-CSF Antibody (4D6C4), mAb, Mouse	40 μg	$50

Full Name

GM-CSF Antibody (4D6C4), mAb, Mouse

Abbreviated name-1

Mouse Anti-huGM-CSF mAb;

Documents
Document-MSDS: 9490_20090304031147.PDF (PDF)
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Reference

Description

Granulocyte-Macrophage-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. GM-CSF also acts upon mature macrophages, eosinophils and neutrophils to stimulate various functional activities. GM-CSF is an acidic glycoprotein (23 kD mouse, 18-22 kD human) which binds to high affinity receptors on GM-CSF sensitive cells. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific. Other growth factors and CSFs modulate receptor binding or actions of GM-CSF.

Ig Subclass

Mouse IgG1, k

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

This product reacts with human GM-CSF. It has not yet been tested with other species.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

GM-CSF full length recombinant protein

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Fusion Partner

Spleen cells fused with SP2/0-Ag14 mouse myeloma cells

Conjugation

Unconjugated

Host Species

Mouse

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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