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Doublecortin Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01303-200	Doublecortin Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01303-40	Doublecortin Antibody, pAb, Rabbit	40 μg	$50

Full Name

Doublecortin Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-Doublecortin pAb;

Documents
Document-MSDS: 9566_20090410035007.PDF (PDF)
Figures
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Reference

Description

Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. It seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Doublecortin protein may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. As a cytoskeletal protein which affects neuronal migration by regulating the stability of microtubules, Doublecortin protein may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. Doublecortin is likely to work with LIS-1 in an overlapping, but distinct, signaling pathway that promotes neuronal migration.

GenScript Doublecortin Antibody, pAb, Rabbit is developed in Rabbit using a KLH-coupled synthetic peptide from within residues 230-280 of human Doublecortin protein (Swiss Prot: O43602).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Doublecortin Antibody, pAb, Rabbit detects endogenous levels of human, mouse and rat Doublecortin protein.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide from within residues 230-280 of human Doublecortin protein (Swiss Prot: O43602).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Predicted Band Size

49 kD

Observed Band Size

49 kD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human, mouse and rat. Reactivity to other species is not tested yet.

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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