Catalog Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » antibody : Rabbit Anti-TDP43 Polyclonal Antibody
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A01304-40
40 μg
$79 $47
TDP43 Antibody, pAb, Rabbitcustom antibody production
TDP43 Antibody, pAb, Rabbit
Full Name TDP43 Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-TARDBP antibody;
Documents
TDP43 Antibody, pAb, RabbitDocument-MSDS: 9570_20090410040542.PDF (PDF)
Figures
TDP43 Antibody, pAb, Rabbit zoomTDP43 Antibody, pAb, Rabbit
Description
TDP43 (known as TARDBP, TAR DNA-binding protein 43) is a DNA and RNA-binding protein which regulates transcription and splicing. The deduced 414-amino acid TDP43 contains a ribonucleoprotein (RNP)-binding domain and a glycine-rich region. TDP43 protein is located at cell nuclear in normal condition. TDP43 promotes skipping of exon 9 of the CFTR gene and also inhibits human CFTR exon 9 splicing. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43, known as pathologic TDP43, is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and amyotrophic lateral sclerosis (ALS). Under pathologic conditions in FTLD-U, TDP43 is likely to be eliminated from nuclei of ubiquitinated inclusion-bearing neurons, a consequence of which may be a loss of TDP43 nuclear functions. TDP43 has been implicated in AIDS disease.

GenScript Rabbit Anti-TDP43 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from within residues 250-300 of human TDP43 protein (Swiss Prot: Q13148).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-TDP43 Polyclonal Antibody detects endogenous levels of human, mouse and rat TDP43 protein.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide from within residues 250-300 of human TDP43 protein (Swiss Prot: Q13148).
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Predicted Band Size
45 kD
Observed Band Size
45 kD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human, mouse and rat. Reactivity to other species is not tested yet.
Host Species
Rabbit
Antigen Species
Human
Note
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
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