| Full Name |
Goat Anti-Mouse IgG Antibody (H&L) [FITC], pAb
|
Abbreviated name-1 |
Goat Anti-Mouse IgG (H&L) [FITC] pAb; |
|
Description |
Goat Anti-Mouse IgG (H&L) [FITC] Polyclonal Antibody is developed in goat using purified mouse IgG whole molecule. This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to FITC.
Goat Anti-Mouse IgG (H&L) [FITC] Polyclonal Antibody is suitable for immunocytochemistry, immunohistochemistry and flow cytometry analysis as well as other antibody based fluorescent assays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: 4.2 moles FITC per mole of Goat IgG. |
Ig Subclass |
Goat IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
This antibody reacts with mouse IgG heavy and light chains. |
Concentration |
1 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide |
Immunogen |
Purified mouse IgG whole molecule |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with 1 ml of deionized water (or equivalent) to an antibody concentration of 1 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Flow cytometry: 1:200-1:500
Immunocytochemistry: 1:200-1:500
Immunohistochemistry: 1:100-1:300
Other Applications: user-optimized |
Conjugation |
Fluorescein isothiocyanate (FITC) |
Host Species |
Goat |
Antigen Species |
Mouse |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
|
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Document-MSDS: 9675_20090427230411.PDF (PDF)