GST-tag Antibody [HRP], pAb, Goat- GenScript, AAALAC International Accredited Animal Facility

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Cat. No.	Name	Size	Price
A01380-40	GST-tag Antibody [HRP], pAb, Goat	40 μg	$87
A01380-100	GST-tag Antibody [HRP], pAb, Goat	100 ug	$218

GST-tag Antibody [HRP], pAb, Goat

Full Name

GST-tag Antibody [HRP], pAb, Goat

Abbreviated name-1

Goat Anti-GST-tag [HRP] pAb;

Documents

Document-MSDS: 9600_20090420042212.PDF (PDF)

Figures

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Reference

Description

Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished by using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by introducing site-specific proteases. Antibodies directed against GST can detect both the fusion protein during purification and the cleavage of GST from the protein of interest.

GenScript Goat Anti-GST-tag [HRP] Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum. The antibody is highly purified by immunoaffinity chromatography from goat antiserum and is then conjugated to horseradish peroxidase.

Ig Subclass

Goat IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Goat Anti-GST-tag [HRP] Polyclonal Antibody detects GST fusion proteins in expression systems including bacterial expression system, mammalian expression system and others.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.

Immunogen

Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-1 µg/ml
Other Applications: user-optimized

Conjugation

Horseradish peroxidase (HRP)

Host Species

Goat

Technical Manual

ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)

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