Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants.
GenScript Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
GenScript Rabbit Anti-GFP Polyclonal Antibody reacts with GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv.
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.5-1 µg/ml Immunoprecipitation (IP): 2-10 µg/mg of lysate Other Applications: user-optimized
Western blot analysis of GFP fusion protein using 1 µg/ml Rabbit Anti-GFP Polyclonal Antibody (GenScript, A01388) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 27 KD Observed Size: 27 KD
Western blot analysis of GFPuv fusion protein using 1 µg/ml Rabbit Anti-GFP Polyclonal Antibody (GenScript, A01388) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 27 KD Observed Size: 27 KD
Western blot analysis of YFP fusion protein using 1 µg/ml Rabbit Anti-GFP Polyclonal Antibody (GenScript, A01388) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 40 KD Observed Size: 40 KD
Western blot analysis of immunoprecipitates from lysate expressing GFP using Rabbit Anti-GFP Polyclonal Antibody (GenScript, A01388). Lane 1: Input control material for lysate expressing GFP protein Lane 2: Negative control – IP with isotype control antibody (GenScript, A01008) Lane 3: Immunprecipitation with Rabbit Anti-GFP Polyclonal Antibody (GenScript, A01388).
Kulasekara BR., et al. c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility. Elife. 2013 Dec;2(0):e01402.
Wang S., et al. Nudel/NudE and Lis1 promote dynein and dynactin interaction in the context of spindle morphogenesis. Mol Biol Cell. 2013 Sep;24(22):3522-3533.
Martínez-Vieyra IA., et al. A Role For Β-Dystroglycan In The Organisation And Structure Of The Nucleus In Myoblasts. Biochim Biophys Acta. 2013 Mar;S0167-4889(3):698-711.
Kwong RW., et al. Evidence for a role of tight junctions in regulating sodium permeability in zebrafish (Danio rerio) acclimated to ion-poor water. J Comp Physiol B. 2013 Feb;183(2):203-213.
Zhaofei Li and Gary W. Blissard. Cellular Vps4 Is Required For Efficient Entry And Egress Of Budded Virions Of Autographa Californica Multiple Nucleopolyhedrovirus. J Virol. 2012 Jan;86(1):459-72.
Muzammel Haque and Konstantin G. Kousoulas. The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Binds to HIF-1α and Causes Its Degradation via the Proteasome Pathway. J Virol. 2013 Feb;87(4):2164 - 2173.
Zhang H., et al. YAP accelerates AÎ²25â€“35-induced apoptosis through upregulation of Bax expression by interaction with p73. Apoptosis. 2011 Aug;16(8):808-821.
FragniÃ¨re C., et al. Salicylic acid and its location in response to biotic and abiotic stress. FEBS Lett. 2011 Jun;585(12):1847-52.
BarcelÓ C., et al. Oncogenic K-ras segregates at spatially distinct plasma membrane signaling platforms according to its phosphorylation status. J Cell Sci. 2013 Oct;126(Pt 20):4553-9.
AD Land, et al. Functional Domain Analysis of the Cell Division Inhibitor EzrA. PLoS One. 2014July;9(7):e102616