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GFP Antibody, pAb, Rabbit



Cat. No.
Name
Size   
Price
Selected
A01388-200
GFP Antibody, pAb, Rabbit
200 μg(5x40μg)
$225
A01388-40
GFP Antibody, pAb, Rabbit
40 μg
$50
GFP Antibody, pAb, Rabbitcustom antibody production
GFP Antibody, pAb, Rabbit
Full Name GFP Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-GFP pAb;
Documents
GFP Antibody, pAb, RabbitDocument-MSDS: 9764_20090507205032.PDF (PDF)
Figures
GFP Antibody, pAb, Rabbit zoomGFP Antibody, pAb, Rabbit
GFP Antibody, pAb, Rabbit zoomGFP Antibody, pAb, Rabbit
GFP Antibody, pAb, Rabbit zoomGFP Antibody, pAb, Rabbit
GFP Antibody, pAb, Rabbit zoomGFP Antibody, pAb, Rabbit
Reference
Description
Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants.

GenScript Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-GFP Polyclonal Antibody reacts with GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Purified recombinant full-length GFP protein
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other Applications: user-optimized
Conjugation
Unconjugated
Host Species
Rabbit
Antigen Species
Human
Technical Manual
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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1
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