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FOXA1 Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01389-200	FOXA1 Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01389-40	FOXA1 Antibody, pAb, Rabbit	40 μg	$50

Full Name

FOXA1 Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-FOXA1 pAb;

Documents
Document-MSDS: 9734_20090504013651.PDF (PDF)
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Reference

Description

Forkhead box protein A1 (FOXA1) is an evolutionarily conserved winged helix transcription factor that was traditionally considered to be involved in embryonic development and cell differentiation. It plays a pro-apoptotic role by inhibiting the expression of anti-apoptotic gene bcl2. FOXA1 plays a critical role in defining the ERalpha cistrome in MCF7 cells. It may serve as a therapeutic target for breast cancer. Despite the ability of FOXA1 to bind nucleosomes, its differential binding to chromatin sites is dependent on the distribution of histone H3 lysine 4 dimethylation. In addition, FOXA1 regulate multiple phases of midbrain dopaminergic neuron development.

GenScript Rabbit Anti-FOXA1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from within residues 200-250 of human FOXA1 (Swiss Prot: P55317).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-FOXA1 Polyclonal Antibody detects endogenous levels of human and mouse FOXA1 protein. It is predicted to react with rat FOXA1 protein according to sequence homology. This antibody also recognizes FOXA3.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide within from residues 200-250 of human FOXA1 (Swiss Prot: P55317).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
Other Applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human and mouse. This product has not been tested with other species yet.

Host Species

Rabbit

Antigen Species

Human

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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