| Full Name |
MELK Antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-MELK pAb; |
|
Description |
MELK (Maternal Embryonic Leucine zipper Kinase, Protein kinase PK38) is one member of the Snf1/AMPK related kinase family of serine/threonine kinases. MELK kinase contains an N-terminal serine/threonine kinase domain that includes all 12 typical catalytic subdomains, and a KA1 (kinase-associated) domain. It is implicated in stem cell renewal, cell cycle progression, and pre-m-RNA splicing. The kinase is also a marker for self-renewing multipotent neural progenitors in the developing forebrain. MERK phosphorylates zinc finger protein 622 (ZNF622) and may contribute to its redirection to the nucleus. MERK may be involved in the inhibition of spliceosome assembly during mitosis.
GenScript Rabbit Anti-MELK Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human MELK. (Swiss Prot: Q14680). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-MELK Polyclonal Antibody detects endogenous levels of human and mouse MELK protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from C-terminal of human MELK (Swiss Prot: Q14680). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human and mouse. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
|
Document-MSDS: 9737_20090504015544.PDF (PDF)
