|
TrkA Antibody, pAb, Rabbit  |
|
|
| Full Name |
TrkA Antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-TrkA pAb; |
|
Description |
TrkA is one member of Trk receptor tyrosine kinase family that consists of TrkA, TrkB and TrkC. TrkA plays an important role in proliferation and maturation of the nervous system. TrkA phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. TrkA kinase activity depends on phosphorylation of residues Tyr674/675 within catalytic domain. TrkA signals growth arrest and differentiation of cells originating from the neural crest, and can serve as a prognostic marker in neuroblastomas.
GenScript Rabbit Anti-TrkA Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human TrkA (Swiss Prot: P04629). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-TrkA Polyclonal Antibody detects human TrkA protein. It is predicted to react with mouse and rat according to sequence homology. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from C-terminal of human TrkA (Swiss Prot: P04629). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
|
 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
|
Document-MSDS: 9833_20090524221214.PDF (PDF)