Catalog Products » Antibodies » THE™ Elite Antibodies » THETM alpha Tubulin Antibody, mAb, Mouse
Cat. No.
Name
Size   
Price
Selected
A01410-100
100 μg
$225
A01410-40
40 μg
$108 $65
THETM alpha Tubulin Antibody, mAb, Mousecustom antibody production
THE<sup>TM</sup> alpha Tubulin Antibody, mAb, Mouse
Full Name THETM alpha Tubulin Antibody, mAb, Mouse
Abbreviated name-1
α-tubulin mAb;
Documents
THE<sup>TM</sup> alpha Tubulin Antibody, mAb, MouseDocument-MSDS: 10771_20090907231749.PDF (PDF)
Figures
THE<sup>TM</sup> alpha Tubulin Antibody, mAb, Mouse zoomTHE<sup>TM</sup> alpha Tubulin Antibody, mAb, Mouse
THE<sup>TM</sup> alpha Tubulin Antibody, mAb, Mouse zoomTHE<sup>TM</sup> alpha Tubulin Antibody, mAb, Mouse
THE<sup>TM</sup> alpha Tubulin Antibody, mAb, Mouse zoomTHE<sup>TM</sup> alpha Tubulin Antibody, mAb, Mouse
Description
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to α-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.
Ig Subclass
IgG2b,κ
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
THETM alpha Tubulin Antibody, mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH
Clone ID
1F4E3
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Conjugation
Unconjugated
Host Species
Mouse
Note
GenScript can offer this product per customer's reuqest including product size, buffer components,etc.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
  • 10K000219_A01410_COA.pdf
  • 13D000613_A01410_COA.pdf
  • 13S000674_A01410_COA.pdf
  • Citation
    Kuo HP., et al. Epigenetic Roles of MLL Oncoproteins Are Dependent on NF-κB. Cancer Cell. 2013 Oct;24(4):423-37

    Chen HW., et al. Inhibitory and combinatorial effect of diphyllin, a v-ATPase blocker, on influenza viruses. Antiviral Res. 2013 Sep;99(3):371-82.

    Amita M., et al. Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4. Proc Natl Acad Sci U S A. 2013 Mar;110(13):E1212 - E1221.

    Schmid M., et al. Apicomplexan Parasite, Eimeria falciformis, Co-opts Host Tryptophan Catabolism for Life Cycle Progression in Mouse. J Biol Chem. 2012 Jun;287(24):20197-207.

    Lakshmana MK., et al. Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain. FASEB J. 2012 May;26(5):2072-83.

    Liberman R., et al. Regulated Assembly of the V-ATPase is Increased During Cluster Disruption-Induced Maturation of Dendritic Cells Through a PI-3 Kinase/mTOR-dependent Pathway. J Biol Chem. 2014 Jan;289(3):1355-63.

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