Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to α-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
THETM alpha Tubulin Antibody, mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species.
A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH
Protein A affinity column
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Immunofluorescent staining: 1 µg/ml Other applications: user-optimized
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
GenScript can offer this product per customer's reuqest including product size, buffer components,etc.
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