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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM alpha Tubulin Antibody, mAb, Mouse
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THETM alpha Tubulin Antibody, mAb, Mouse


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Cat. No.
Product Name
Size
Price
Quantity
A01410-40 THETM alpha Tubulin Antibody, mAb, Mouse
40 μg
$108.00
A01410-100 THETM alpha Tubulin Antibody, mAb, Mouse
100 μg
$225.00

 


Abbreviated name-1 α-tubulin mAb;
Description Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to α-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.
Immunogen A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH
Host Species Mouse
Conjugation Unconjugated
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Method Protein A affinity column

Formulation 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Clone ID 1F4E3
Ig Subclass IgG2b,κ
Specificity THETM alpha Tubulin Antibody, mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can offer this product per customer's reuqest including product size, buffer components,etc.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse
THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

Comparison of THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410) with Mouse Anti-alpha Tubulin mAb (Competitor A) by Western blot.
A: THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
B: Mouse Anti-alpha Tubulin Monoclonal Antibody (Clone: DM1A, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse
THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

Western blot analysis of cell and tissue lysates using THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse
THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

Immunofluorescence analysis of 293 cells
Primay antibody: 1 μg/ml THETM Anti-alpha tubulin Monoclonal Antibody (Mouse) (GenScript, A01410)
Secondary antibody: Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody, MAb, Mouse


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You can visit "Citations Database" for more citations. Schmid M., et al. Apicomplexan Parasite, Eimeria falciformis, Co-opts Host Tryptophan Catabolism for Life Cycle Progression in Mouse. J Biol Chem. 2012 Jun;287(24):20197-207.

You can visit "Citations Database" for more citations. Lakshmana MK., et al. Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain. FASEB J. 2012 May;26(5):2072-83.

You can visit "Citations Database" for more citations. Liberman R., et al. Regulated Assembly of the V-ATPase is Increased During Cluster Disruption-Induced Maturation of Dendritic Cells Through a PI-3 Kinase/mTOR-dependent Pathway. J Biol Chem. 2014 Jan;289(3):1355-63.

You can visit "Citations Database" for more citations. Y Shibuya, et al. Inhibiting ACAT1/SOAT1 in Microglia Stimulates Autophagy-Mediated Lysosomal Proteolysis and Increases Aβ1–42 Clearance. J Neurosci. 2014Qct.;34(43):14484-501

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