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GAP-43 Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01414-200	GAP-43 Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01414-40	GAP-43 Antibody, pAb, Rabbit	40 μg	$50

Full Name

GAP-43 Antibody, pAb, Rabbit

Abbreviated name-1

GAP 43 antibody;

Documents
Document-MSDS: 9942_20090614232945.PDF (PDF)
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Reference

Description

GAP-43 has been termed as a growth or plasticity protein, because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is considered to be a crucial component involved in effective regenerative response in nervous system. Its phosphorylation by protein kinase C correlates with long-term potentiation. It is used widely to specifically label injured neurons and to score neuronal regeneration.
GenScript Rabbit Anti-GAP-43 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human GAP-43 (Swiss Prot: P17677).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-GAP-43 Polyclonal Antibody detects endogenous levels of mouse and rat GAP-43. It is predicted to react with human GAP-43 according to sequence homology.

Positive Control: Mouse brain and rat brain lysates

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Immunogen

KLH-coupled synthetic peptide from C-terminal of human GAP-43 (Swiss Prot: P17677).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Mouse and rat. This product has not been tested with other species yet.

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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