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NCAM Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01415-200	NCAM Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01415-40	NCAM Antibody, pAb, Rabbit	40 μg	$50

Full Name

NCAM Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-NCAM pAb;

Documents
Document-MSDS: 9859_20090526224103.PDF (PDF)
Figures
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Reference

Description

NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein. It contains five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Alternative splicing gives rise to different cytoplasmic domains. NCAM is involved in neuronal attachment, neurite extension, and cell-cell interactions through homo and heterophilic interactions. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. It is expressed on most neuroectodermal derived cell lines, tissues, and neoplasm.

GenScript Rabbit Anti-NCAM Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within cytoplasmic residues 780-830 of human NCAM (Swiss Prot: P13591).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-NCAM Polyclonal Antibody detects endogenous levels of rat and mouse NCAM. It is predicted to react with human NCAM according to sequence homology.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide within cytoplasmic residues 780-830 of human NCAM (Swiss Prot: P13591).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Rat and mouse. This product has not been tested with other species yet.

Host Species

Rabbit

Antigen Species

Human

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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