Nurr1 is a transcription factor and belongs to the Nur77 family of orphan receptors. This family of transcription factors plays key roles in the development and the effector functions of T lymphocytes. Nurr1 promote signaling via its heterodimerization partner, the retinoid X receptor (RXR). Nurr1 protein is expressed in the embryonic ventral midbrain and it is critical for the development of dopamine neurons. In addition, Nurr1 has been implicated in the maintenance of adult dopaminergic neurons. GenScript Nurr1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 310~360 of human Nurr1 protein (Swiss Prot: P43354).
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
GenScript Nurr1 Antibody, pAb, Rabbit detects human Nurr1 protein. Predicted to react with rat and mouse Nurr1 protein according to sequence homology.
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 1-2 µg/ml Other Applications: user-optimized
Western blot analysis of extracts from HEK293 cells transfected with human Nurr1 for 48 hr (lane 1) and 72hr (lane 2) using Nurr1 Antibody, pAb, Rabbit (GenScript, A01425, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 80 kD
Observed Size: 80 kD