THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse
|
THE TM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse  |
|
|
![THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], mAb, Mouse](/images/cgi2007/table2.gif "THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], mAb, Mouse")
| Full Name |
THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse
|
Abbreviated name-1 |
anti Flag-tag mAb; anti DYKDDDDK-tag mAb |
|
Description |
GenScript THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM DYKDDDDK Tag Antibody, mAb, Mouse (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins. |
Ig Subclass |
IgG2b, k |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
A synthetic peptide (DYKDDDDK) coupled to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
|
Fusion Partner |
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
Conjugation |
Biotin |
Host Species |
Mouse |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
|
Related Products
 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
|
![THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], mAb, Mouse](/images/download_PDF.jpg "THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], mAb, Mouse")
Document-MSDS: 3739_20080118034804.PDF (PDF)![THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], mAb, Mouse](/ssl-bin/site2/show_image.cgi?op=10211_20090721015900.JPG "THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], mAb, Mouse")
