PDI Antibody, pAb, Rabbit
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PDI Antibody, pAb, Rabbit  |
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| Full Name |
PDI Antibody, pAb, Rabbit
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Abbreviated name-1 |
PDI A1 antibody; ER Marker |
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Description |
Protein Disulphide Isomerase (PDI) is a microsomal enzyme which locates in endoplasmic reticulum lumen. The enzyme catalyzes the formation and isomerization of intermolecular disulphide-bond that stabilizes three dimensional structure of many extracellular proteins. The upregulation of PDI represents an adaptive response to the accumulation of immature and denatured proteins which cause ER dysfunction in neurodegenerative disorders.
GenScript Rabbit Anti-PDI Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 100-150 of human PDI (Swiss Prot: P07237).
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-PDI Polyclonal Antibody detects endogenous levels of human, mouse, and rat PDI.
Positive Control: K562, HepG2, A431, Hela, Ramos, mouse brain and rat brain lysates |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Immunogen |
KLH-coupled synthetic peptide within residues 100-150 of human PDI (Swiss Prot: P07237). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other Applications: user-optimized
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Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, and rat. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Related Products
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
|
Document-MSDS: 9938_20090611060605.PDF (PDF)
