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Parvalbumin Antibody, pAb, Rabbit  |
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| Full Name |
Parvalbumin Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-PVALB antibody; |
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Description |
Parvalbumin (PV) is a calcium binding protein. It belongs to a larger group of EF hand proteins and consists of a single, unbranched chain of linked amino acids. Parvalbumin participates in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. And it is implicated in maintaining the balance between excitation and inhibition in the cortex as well as in the hippocampus. It is also expressed in specific muscle fibers and fast-firing neurons.
GenScript Rabbit Anti-Parvalbumin Polyclonal Antibody is developed in rabbit using purified His-tagged human Parvalbumin (Swiss Prot: P20472) full length recombinant protein. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Parvalbumin Polyclonal Antibody detects endogenous levels of human, mouse, and rat Parvalbumin.
Positive Control: Mouse brain and rat brain.
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Immunogen |
Purified His-tagged human Parvalbumin (Swiss Prot: P20472) full length recombinant protein. |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other Applications: user-optimized
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Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, and rat. This product has not been tested for other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 10111_20090709090403.PDF (PDF)
