Catalog Products » Antibodies » Primary Antibodies » Cell Marker Antibodies » Lamin A+C Antibody, pAb, Rabbit
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A01455-40
40 μg
$79 $47
A01455-200
200 μg(5x40μg)
$275
Lamin A+C Antibody, pAb, Rabbitcustom antibody production
Lamin A+C Antibody, pAb, Rabbit
Full Name Lamin A+C Antibody, pAb, Rabbit
Abbreviated name-1
Lamin A/C antibody;
Documents
Lamin A+C Antibody, pAb, RabbitDocument-MSDS: 10233_20090723064826.PDF (PDF)
Figures
Lamin A+C Antibody, pAb, Rabbit zoomLamin A+C Antibody, pAb, Rabbit
Lamin A+C Antibody, pAb, Rabbit zoomLamin A+C Antibody, pAb, Rabbit
Citations
Ke H.,et al. CYLD Inhibits Melanoma Growth and Progression through Suppression of the JNK/AP-1 and β1-Integrin Signaling Pathways.J Invest Dermatol.2013Jan;133(1):221-9

Description
Lamins are structural protein components of the nuclear lamina which contains 3 members: Lamin A, B and C in mammalian cells. Lamin A and lamin C are generated by alternative splicing from the same gene and share complete identity for the first 566 amino acids. Lamin A interacts with transcription factor SREBP1 via its C-terminal domain. The lamin A/C deficiency is probably associated with both defective nuclear mechanics and impaired transcriptional activation. The lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation.

GenScript Rabbit Anti-Lamin A+C Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-450 of human Lamin A (Swiss Prot: P02545).
Ig Subclass
Rabbit IgG
Storage
GenScript Rabbit Anti-Lamin A+C Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.
Specificity
GenScript Rabbit Anti-Lamin A+C Polyclonal Antibody detects endogenous levels of human and mouse Lamin A and Lamin C. It is predicted to react with rat Lamin A and Lamin C according to sequence homology.
Positive Control: Hela, A431, Lovo and NIH/3T3
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide within residues 400-450 of human Lamin A (Swiss Prot: P02545).
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other Applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human and mouse. This product has not been tested for other species yet.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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