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Lamin A+C Antibody, pAb, Rabbit  |
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| Full Name |
Lamin A+C Antibody, pAb, Rabbit
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Abbreviated name-1 |
Lamin A/C antibody; |
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Description |
Lamins are structural protein components of the nuclear lamina which contains 3 members: Lamin A, B and C in mammalian cells. Lamin A and lamin C are generated by alternative splicing from the same gene and share complete identity for the first 566 amino acids. Lamin A interacts with transcription factor SREBP1 via its C-terminal domain. The lamin A/C deficiency is probably associated with both defective nuclear mechanics and impaired transcriptional activation. The lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation.
GenScript Rabbit Anti-Lamin A+C Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-450 of human Lamin A (Swiss Prot: P02545).
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Ig Subclass |
Rabbit IgG |
Storage |
GenScript Rabbit Anti-Lamin A+C Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
GenScript Rabbit Anti-Lamin A+C Polyclonal Antibody detects endogenous levels of human and mouse Lamin A and Lamin C. It is predicted to react with rat Lamin A and Lamin C according to sequence homology.
Positive Control: Hela, A431, Lovo and NIH/3T3
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide within residues 400-450 of human Lamin A (Swiss Prot: P02545). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other Applications: user-optimized
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Conjugation |
Unconjugated |
Species Reactivity |
Human and mouse. This product has not been tested for other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 10233_20090723064826.PDF (PDF)
