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Human RANTES Antibody (4A10F2), mAb, Mouse

Cat. No.	Name	Size	Price
A01479-200	Human RANTES Antibody (4A10F2), mAb, Mouse	200 μg(5x40μg)	$225
A01479-40	Human RANTES Antibody (4A10F2), mAb, Mouse	40 μg	$50

Full Name

Human RANTES Antibody (4A10F2), mAb, Mouse

Abbreviated name-1

Mouse anti RANTES mAb; Mouse anti CCL5 mAb

Documents
Document-MSDS: 10592_20090827075613.PDF (PDF)
Figures
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Reference

Description

RANTES is a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils and binds to CCR1, CCR3, CCR4, and CCR5. It is also one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). Induced by mitogens, it is T cell and macrophage specific and belongs to the intercrine beta (chemokine CC) family.
GenScript Human RANTES Antibody (4A10F2), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified full length human recombinant RANTES protein (Swiss Prot: P13501).

Ig Subclass

IgG2b,κ

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Human RANTES Antibody (4A10F2), mAb, Mouse detects human RANTES protein.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

Purified full length human recombinant RANTES protein (Swiss Prot: P13501)

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 0.5-10 μg/ml
ELISA Detection: 0.05-0.2 μg/ml
Recommended antibody pairing for sandwich immunoassay:

Western Blot: 0.5-1.0 μg/ml
Other Applications: user-optimized

Fusion Partner

Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Conjugation

Unconjugated

Species Reactivity

Human. Reactivity to other species has not been tested yet.

Host Species

Mouse

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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