Catalog Products » Antibodies » Primary Antibodies » Cytokine and Growth Factor Antibodies » IL-1 beta Antibody (6B1G11), mAb, Mouse
GenScript Share
Cat. No.
Name
Size   
Price
Selected
A01485-40
40 μg
$79 $47
A01485-200
200 μg(5x40μg)
$275
IL-1 beta Antibody (6B1G11), mAb, Mousecustom antibody production
IL-1 beta Antibody (6B1G11), mAb, Mouse
Full Name IL-1 beta Antibody (6B1G11), mAb, Mouse
Abbreviated name-1
H1 antibody; LAF antibody
Documents
IL-1 beta Antibody (6B1G11), mAb, MouseDocument-MSDS: 10775_20090907232710.PDF (PDF)
Figures
IL-1 beta Antibody (6B1G11), mAb, Mouse zoomIL-1 beta Antibody (6B1G11), mAb, Mouse
IL-1 beta Antibody (6B1G11), mAb, Mouse zoomIL-1 beta Antibody (6B1G11), mAb, Mouse
Citations
Description
Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which subsequently proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes. It is also released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta), not only bind to the same cell surface receptor and elicit nearly identical biological responses but also share 25% homology in their amino acid sequence.
Ig Subclass
Mouse IgG2a,κ
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
No cross-reactivity with IL-1α, IL-4, IL-6, IL-8, IL-18 are detected.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Human IL-1β full length recombinant protein
Clone ID
6B1G11
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other: user-optimized
Fusion Partner
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Conjugation
Unconjugated
Host Species
Mouse
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.