Catalog Products » Antibodies » Epitope Tag Antibodies » KLH Antibody, mAb, Mouse
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A01486-40
40 μg
$79 $47
KLH Antibody, mAb, Mousecustom antibody production
KLH Antibody, mAb, Mouse
Full Name KLH Antibody, mAb, Mouse
Abbreviated name-1
Keyhole Limpet Hemocyanin mAb ; KLH
Documents
KLH Antibody, mAb, MouseDocument-MSDS: 10786_20090908020833.PDF (PDF)
Figures
KLH Antibody, mAb, Mouse zoomKLH Antibody, mAb, Mouse
Description
Keyhole limpet hemocyanin (KLH) is a high molecular-weight and copper-containing protein. It is found in the hemolymph of sea mollusk Megathura crenulata. The KLH protein is potently immunogenic yet safe in human and is therefore extensively used as a carrier protein in the production of antibodies for research as well as in other biotechnology and therapeutic applications.
Ig Subclass
Mouse IgG1,κ
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
No cross-reactivity with BSA and OVA are detected
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Full keyhole limpet hemocyanin
Clone ID
3C1G5
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Dot blot: 0.1-1.0 µg/ml
Other: user-optimized
Fusion Partner
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Conjugation
Unconjugated
Host Species
Mouse
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
Citation
Tawk L., et al. A key role for Plasmodium subtilisin-like SUB1 in egress of malaria parasites from host hepatocytes. J Biol Chem. 2013 Nov;288(46):33336-46.

Ling Xiao., et al. KIBRA Regulates Hippo Signaling Activity via Interactions with Large Tumor Suppressor Kinases. J Biol Chem. 2011 Mar;286(10):7788 - 7796.

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