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KLH Antibody, mAb, Mouse

Cat. No.	Name	Size	Price
A01486-200	KLH Antibody, mAb, Mouse	200 μg(5x40μg)	$225
A01486-40	KLH Antibody, mAb, Mouse	40 μg	$50

Full Name

KLH Antibody, mAb, Mouse

Abbreviated name-1

Keyhole Limpet Hemocyanin mAb ; KLH

Documents
Document-MSDS: 10786_20090908020833.PDF (PDF)
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Reference

Description

Keyhole limpet hemocyanin (KLH) is a high molecular-weight and copper-containing protein. It is found in the hemolymph of sea mollusk Megathura crenulata. The KLH protein is potently immunogenic yet safe in human and is therefore extensively used as a carrier protein in the production of antibodies for research as well as in other biotechnology and therapeutic applications.

Ig Subclass

Mouse IgG1,κ

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

No cross-reactivity with BSA and OVA are detected

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

Full keyhole limpet hemocyanin

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Dot blot: 0.1-1.0 µg/ml
Other: user-optimized

Fusion Partner

Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Conjugation

Unconjugated

Host Species

Mouse

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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