||Keyhole Limpet Hemocyanin mAb ; KLH
||Keyhole limpet hemocyanin (KLH) is a high molecular-weight and copper-containing protein. It is found in the hemolymph of sea mollusk Megathura crenulata. The KLH protein is potently immunogenic yet safe in human and is therefore extensively used as a carrier protein in the production of antibodies for research as well as in other biotechnology and therapeutic applications.
||Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
||Protein A affinity column
||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
||Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
||No cross-reactivity with BSA and OVA are detected
||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Dot blot: 0.1-1.0 µg/ml
Western Blot Protocol(pdf)
Tawk L., et al. A key role for Plasmodium subtilisin-like SUB1 in egress of malaria parasites from host hepatocytes. J Biol Chem. 2013 Nov;288(46):33336-46.
Ling Xiao., et al. KIBRA Regulates Hippo Signaling Activity via Interactions with Large Tumor Suppressor Kinases. J Biol Chem. 2011 Mar;286(10):7788 - 7796.
Ahrman E, et al. Novel COMP Neoepitopes Identified in Synovial Fluids from Patients with Joint Diseases using Affinity Chromatography and Mass Spectrometry. J Biol Chem. 2014June;