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 Name
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A01490
100 μg
$335
THETM alpha Tubulin Antibody [HRP], mAb, Mousecustom antibody production
THE<sup>TM</sup> alpha Tubulin Antibody [HRP], mAb, Mouse
Full Name THETM alpha Tubulin Antibody [HRP], mAb, Mouse
Abbreviated name-1
 THETM Anti-α-tubulin [HRP] mAb (mouse);
Documents
THE<sup>TM</sup> alpha Tubulin Antibody [HRP], mAb, MouseDocument-MSDS: 11241_20091113045008.PDF (PDF)
Figures
THE<sup>TM</sup> alpha Tubulin Antibody [HRP], mAb, Mouse zoomTHE<sup>TM</sup> alpha Tubulin Antibody [HRP], mAb, Mouse
Reference
Description
 THETM α Tubulin Antibody [HRP], mAb, Mouse is HRP conjugated to THETM α Tubulin Antibody [HRP], mAb, Mouse (Cat. No. A01410) purified from mouse ascites by protein A affinity column. The product is a specific and useful tool in studying proper interpretation of western blots.
Ig Subclass
 Mouse IgG2b, κ
Storage
 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
 THETM alpha Tubulin Antibody [HRP], mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species.
Concentration
 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Immunogen
 KLH-coupled synthetic peptide within residues 400-450 of human α-tubulin (Swiss Prot: P68363).
Reconstitution
 Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Applications
 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-0.5 µg/ml
Other applications: user-optimized
Fusion Partner
 Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Conjugation
 Horseradish peroxidase (HRP)
Host Species
 Mouse
Technical Manual
 ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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