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Cytochrome C Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01507-200	Cytochrome C Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01507-40	Cytochrome C Antibody, pAb, Rabbit	40 μg	$50

Full Name

Cytochrome C Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-Cytochrome C polyclonal antibody; CYC antibody

Documents
Document-MSDS: 11110_20091023062524.PDF (PDF)
Figures
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Reference

Description

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. GenScript Rabbit Anti-Cytochrome C Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human Cytochrome C (Swiss Prot: P99999).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-Cytochrome C Polyclonal Antibody detects endogenous levels of human and mouse Cytochrome C. Predicted to react with rat Cytochrome C according to sequence homology.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Immunogen

KLH-coupled synthetic peptide from N-terminal of human Cytochrome C (Swiss Prot: P99999).

Purification Method

Immunoaffinity chromatography.

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

12 KD

Observed Band Size

12 KD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human and mouse.

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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