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THE TM cGMP Antibody, mAb, Mouse  |
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| Full Name |
THETM cGMP Antibody, mAb, Mouse
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Abbreviated name-1 |
THETM cGMP Antibody, mAb, Mouse; |
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Description |
Guanosine 3, 5-cyclic monophosphate (cyclic GMP; cGMP) acts as a secondary messenger much like cyclic AMP. It is generally known to activate intracellular protein kinases in response to the binding of membrane-impermeable peptide hormones to the cell surface. cGMP synthesis is catalyzed by guanylate cyclase (GC), which converts GTP to cGMP. Membrane-bound GC is activated by peptide hormones such as atrial natriuretic factor, while soluble GC is typically activated by nitric oxide (NO) to stimulate cGMP synthesis.
cGMP is also a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. It also relaxes smooth muscle tissues. The roles of cGMP and cAMP may be linked, as evidenced by the fact that some cellular functions are controlled bi-directionally by both cAMP and cGMP. Some functions are stimulated by cGMP and suppressed by cAMP and vice versa.
GenScript THETM cGMP Antibody, mAb, Mouse is developed using 3, 5-cyclic GMP-8-KLH as immunogen.
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Ig Subclass |
Mouse IgG2a, κ |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
3', 5'-cyclic GMP-8-KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.04-0.1 µg/ml
Other: user-optimized
This antibody is derived from immunization of cyclic GMP derivative conjugated to KLH at position 8 of the guanine structure. It may not react well with cGMP conjugated via other positions.
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Fusion Partner |
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells. |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Note |
GenScript can offer this product per customer's reuqest including product size, buffer components,etc. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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Related Products
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 11231_20091113042219.PDF (PDF)