C-Fos Antibody, pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A01512-40
40 μg
$79 $47
C-Fos Antibody, pAb, Rabbit
Full Name C-Fos Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-C-Fos polyclonal antibody; C-Fos antibody
Documents
C-Fos Antibody, pAb, RabbitDocument-MSDS: 11144_20091027040518.PDF (PDF)
Figures
C-Fos Antibody, pAb, Rabbit zoomC-Fos Antibody, pAb, Rabbit
C-Fos Antibody, pAb, Rabbit zoomC-Fos Antibody, pAb, Rabbit
Description
Transcription factor C-Fos is one member of Fos family of nuclear oncogenes which includes c-Fos, FosB, Fos-related antigen 1 (FRA1) and Fos-related antigen 2 (FRA2). C-Fos is a major component of the activator protein-1 (AP-1) transcription factor complex and dimerizes with Jun proteins (c-Jun, JunB and JunD) to form AP-1 complex. AP-1 upregulates transcription of a diverse range of genes containing AP-1 regulatory elements. And phosphorylation of c-Fos on serine 32 and threonine 232 by ERK-5 increases protein stability and nuclear localization. C-Fos probably serves as a genetic regulator for cellular mechanisms mediating neuronal excitability and survival vial regulation of kainic acid receptor GLUR6 and expression of brain-derived neurotrophic factor BDNF.

GenScript Rabbit Anti-C-Fos Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human C-Fos (Swiss Prot: P01100).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-C-Fos Polyclonal Antibody detects endogenous level ofhuman C-Fos protein. Predicted to react with mouse and rat C-Fos according to sequence homology.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Immunogen
KLH-coupled synthetic peptide from N-terminal of human C-Fos (Swiss Prot: P01100).
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Predicted Band Size
65 KD
Observed Band Size
65 KD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other Applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.