Transcription factor C-Fos is one member of Fos family of nuclear oncogenes which includes c-Fos, FosB, Fos-related antigen 1 (FRA1) and Fos-related antigen 2 (FRA2). C-Fos is a major component of the activator protein-1 (AP-1) transcription factor complex and dimerizes with Jun proteins (c-Jun, JunB and JunD) to form AP-1 complex. AP-1 upregulates transcription of a diverse range of genes containing AP-1 regulatory elements. And phosphorylation of c-Fos on serine 32 and threonine 232 by ERK-5 increases protein stability and nuclear localization. C-Fos probably serves as a genetic regulator for cellular mechanisms mediating neuronal excitability and survival vial regulation of kainic acid receptor GLUR6 and expression of brain-derived neurotrophic factor BDNF.
GenScript Rabbit Anti-C-Fos Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human C-Fos (Swiss Prot: P01100).
KLH-coupled synthetic peptide from N-terminal of human C-Fos (Swiss Prot: P01100).
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
GenScript Rabbit Anti-C-Fos Polyclonal Antibody detects endogenous level ofhuman C-Fos protein. Predicted to react with mouse and rat C-Fos according to sequence homology.
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml Flow cytometry: 1-3 µg for 1 x 106 cells Other Applications: user-optimized
Western blot analysis of human C-Fos recombinant protein using 1 µg/ml Rabbit Anti-C-Fos Polyclonal Antibody (GenScript, A01512) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 65 KD Observed Size: 65 KD
Flow cytometric analysis of K562 cells using C-Fos Antibody, pAb, Rabbit (GenScript, A01512; shaded histogram) or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.