Catalog Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » antibody : PI3 Kinase p85 alpha antibody, pAb, Rabbit
Cat. No.
Name
Size   
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Selected
A01524-40
40 μg
$79 $47
A01524-100
100 μg
$198
PI3 Kinase p85 alpha antibody, pAb, Rabbit
Full Name PI3 Kinase p85 alpha antibody, pAb, Rabbit
Abbreviated name-1
p85 alpha antibody;
Documents
PI3 Kinase p85 alpha antibody, pAb, RabbitDocument-MSDS: 11404_20091130000158.PDF (PDF)
Figures
PI3 Kinase p85 alpha antibody, pAb, Rabbit zoomPI3 Kinase p85 alpha antibody, pAb, Rabbit
PI3 Kinase p85 alpha antibody, pAb, Rabbit zoomPI3 Kinase p85 alpha antibody, pAb, Rabbit
Description
Phosphoinositide 3-kinase (PI3K) catalyzes the phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit (p85). The catalytic subunits of PI3K include p110 alpha, p110 beta, p110 gamma, and p110 delta, while the regulatory subunits contain p85 alpha and p85 beta. p85 alpha regulates the catalytic activity of p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain. The p85 alpha has been implicated in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.GenScript PI3 Kinase p85 alpha Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 600–650 of human PI3 Kinase p85 alpha (Swiss Prot: P27986).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript PI3 Kinase p85 alpha Antibody detects endogenous levels of human PI3 Kinase p85 alpha. Predicted to react with mouse and rat PI3 Kinase p85 alpha according to sequence homology.
Positive Control: Ramos
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Immunogen
KLH-coupled synthetic peptide within residues 600-650 of human PI3 Kinase p85 alpha (Swiss Prot: P27986).
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Predicted Band Size
85 KD
Observed Band Size
85 KD
Conjugation
Unconjugated
Species Reactivity
Human
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
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