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IP-10 Antibody (1G8E2), mAb, Mouse

Full Name

Abbreviated name-1

IP-10 Antibody, mAb, Mouse

Documents
MSDS: 20100315211728 (PDF)
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Reference

Description

Interferon-gamma-inducible 10 kD protein (IP10) is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells, and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis, and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP10 to regulate angiogenic activity in cancer and in idiopathic pulmonary fibrosis.
GenScript IP10 Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified recombinant human protein IP10 (Swiss-Prot P02778).

Ig Subclass

IgG2b,κ

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript IP10 Antibody, mAb, Mouse detects human IP10

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Clone

1G8E2

Immunogen

Purified recombinant human protein IP10 (Swiss-Prot: P02778)

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

32 KD

Observed Band Size

32 KD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
ELISA: 0.1-1.0 µg/ml
Recommended pair for sandwich immunoassay:
Capture: A01564, Clone # 1G8E2
Detection: A01561, Clone # 1G12E6; A01562, Clone # 2H11E8; A01563, Clone # 1H7D6
Other Applications: user optimized

Fusion Partner

Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Conjugation

Unconjugated

Species Reactivity

Human. This product has not been test with other species yet.

Host Species

Mouse

Technical Manual

ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)


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