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E-Cadherin antibody, pAb, Rabbit

*This product has been discontinued!*
GenScript E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology. Positive Control: HepG2 and NIH/3T3
A01589
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Overview
Specificity GenScript E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology. Positive Control: HepG2 and NIH/3T3
Host Species Rabbit
Immunogen KLH-coupled synthetic peptide within residues 650–700 of human E-Cadherin (Swiss Prot: P12830)
Species Reactivity Human and mouse
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 0.5-1 µg/ml
Other applications User-optimized
Flow cytometry 1-3 µg for 1 x 106 cells
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Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
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Background
Target Background E-Cadherin is a key cell adhesion molecule located at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. It is a member of cadherin family that includes cadhesin repeats of approximately 100 residues in their extracellular domian. E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is an important molecule in maintaining epithelial integrity. The expression of E-Cadherin might be associated with the invasive potential of epithelial neoplasms. Defects in E-Cadherin are a cause of gastric cancer, also known as hereditary familial diffuse gastric cancer (HDGC). GenScript E-Cadherin Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 650–700 of human E-Cadherin (Swiss Prot: P12830).
Synonyms Cadherin 1 antibody; CD324 antigen antibody
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Wang J, et al. Enhancer of zeste homolog 2 blockade by RNA interference is implicated with inhibited proliferation, invasion and promoted apoptosis in endometrial carcinoma. Oncol Lett. (2018)
Lu M, et al. The high expression of long non-coding RNA PANDAR indicates a poor prognosis for colorectal cancer and promotes metastasis by EMT pathway. J Cancer Res Clin Oncol. (2017)
Zeng J, et al. Knockdown of Long Noncoding RNA CCAT2 Inhibits Cellular Proliferation, Invasion, and Epithelial-Mesenchymal Transition in Glioma Cells. Oncol Res. (2017)
Ihira Kei, et al. EZH2 inhibition suppresses endometrial cancer progression via miR-361/Twist axis. Oncotarget. (2017)
Zheng J, et al. The up-regulation of long non-coding RNA CCAT2 indicates a poor prognosis for prostate cancer and promotes metastasis by affecting epithelial-mesenchymal transition. Biochem Biophys Res Commun. (2016-08)
Y Konno, et al. MicroRNA-101 targets EZH2, MCL-1 and FOS to suppress proliferation, invasion and stem cell-like phenotype of aggressive endometrial cancer cells. Oncotarget. (2014-08)
Dong P, et al. Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer. Carcinogenesis. (2013-11)
Dong P, et al. Mutant P53 Gain-Of-Function Induces Epithelial-Mesenchymal Transition Through Modulation Of The Mir-130B-Zeb1 Axis. Oncogene. (2012-07)