E-Cadherin antibody, pAb, Rabbit
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Cat. No.
Name
Size   
Price
Selected
A01589-40
40 μg
$79 $47
A01589-200
200 μg(5x40μg)
$275
E-Cadherin antibody, pAb, Rabbitcustom antibody production
E-Cadherin antibody, pAb, Rabbit
Full Name E-Cadherin antibody, pAb, Rabbit
Abbreviated name-1
Cadherin 1 antibody; CD324 antigen antibody
Documents
E-Cadherin antibody, pAb, RabbitDocument-MSDS: 11610_20091225034142.PDF (PDF)
Figures
E-Cadherin antibody, pAb, Rabbit zoomE-Cadherin antibody, pAb, Rabbit
E-Cadherin antibody, pAb, Rabbit zoomE-Cadherin antibody, pAb, Rabbit
Citations
Dong P., et al. Mutant P53 Gain-Of-Function Induces Epithelial-Mesenchymal Transition Through Modulation Of The Mir-130B-Zeb1 Axis. Oncogene. 2013 Jul;32(27):3286-95.

Dong P., et al. Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer. Carcinogenesis. 2013 Dec.

Erin N., et al. Differential characteristics of heart, liver, and brain metastatic subsets of murine breast carcinoma. Breast Cancer Res Treat. 2013 Jun;139(3):677-689.

Description
E-Cadherin is a key cell adhesion molecule located at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. It is a member of cadherin family that includes cadhesin repeats of approximately 100 residues in their extracellular domian. E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is an important molecule in maintaining epithelial integrity. The expression of E-Cadherin might be associated with the invasive potential of epithelial neoplasms. Defects in E-Cadherin are a cause of gastric cancer, also known as hereditary familial diffuse gastric cancer (HDGC).
GenScript E-Cadherin Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 650–700 of human E-Cadherin (Swiss Prot: P12830).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology.
Positive Control: HepG2 and NIH/3T3
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide within residues 650–700 of human E-Cadherin (Swiss Prot: P12830)
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other Applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human and mouse
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
1
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