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BRCA2 antibody, pAb, Rabbit  |
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| Full Name |
BRCA2 antibody, pAb, Rabbit
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Abbreviated name-1 |
FACD antibody; |
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Description |
Breast cancer type 2 susceptibility protein (BRCA2) is involved in double-strand break repair and/or homologous recombination. It may participate in S phase checkpoint activation. In addition, It interacts with RAD51 and DSS1 and binds to ubiquitinated FANCD2. It also interacts with PALB2, enabling the recombinational repair and checkpoints functions. Defects in BRCA2 can cause genetic susceptibility to breast cancer (BC), also called susceptibility to familial breast-ovarian cancer type 2 (BROVCA2).
GenScript BRCA2 Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 2480–2530 of human BRCA2 (Swiss Prot: P51587).
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript BRCA2 Antibody detects endogenous levels of human and mouse BRCA2.
Positive Control: HEK293 and Mouse brain
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Immunogen |
KLH-coupled synthetic peptide within residues 2480–2530 of human BRCA2 (Swiss Prot: P51587) |
Purification Method |
Immunoaffinity chromatography. |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
390 KD |
Observed Band Size |
390 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user optimized
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Conjugation |
Unconjugated |
Species Reactivity |
Mouse |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 11679_20100114223352.PDF (PDF)
