EPHB4, also known as Myk1, is one member of the Eph receptor family which makes up the largest subgroup of the receptor tyrosine kinase (RTK) family. EPHB4 protein is involved in the processes of embryogenesis. It may play a role in the differentiation or maintenance of secretory epithelia. EPHB4 is also an attractive target for drug design because of its recognized role in cancer-related angiogenesis. GenScript EPHB4 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human EPHB4 (Swiss Prot: P54760).
KLH-coupled synthetic peptide from N-terminal of human EPHB4 (Swiss Prot: P54760)
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
GenScript EPHB4 Antibody, pAb, Rabbit detects human and mouse endogenous levels of EPHB4. Positive Control: HEK293, Jurkat, and mouse kidney
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 1-2 µg/ml Other Applications: User optimized
Western blot analysis of cell and tissue lysates using EPHB4 Antibody, pAb, Rabbit (GenScript, A01595, 2 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 108 KD Observed Size: 108 KD, 19KD