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SCF antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01596-200	SCF antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01596-40	SCF antibody, pAb, Rabbit	40 μg	$50

Full Name

SCF antibody, pAb, Rabbit

Abbreviated name-1

SCF Antibody;

Documents
Document-MSDS: 11590_20091224043234.PDF (PDF)
Figures
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Reference

Description

Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis, and fertility. It exerts its activity at the early stages of hematopoiesis. Specifically, SCF stimulates the proliferation of mast cell and mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. In addition, SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways.
GenScript SCF Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human SCF (Swiss Prot: P21583).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.
Positive Control: Ramos cell, mouse kidney tissue lysate

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide from N-terminal of human SCF (Swiss Prot: P21583)

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

33 KD

Observed Band Size

33 KD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user optimized

Conjugation

Unconjugated

Species Reactivity

Human and mouse

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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