Catalog Products » Antibodies » antibody : IL-8 Antibody (1A7C8), mAb, Mouse
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A01605-40
40 μg
$79 $47
A01605-200
200 μg(5x40μg)
$275
IL-8 Antibody (1A7C8), mAb, Mousecustom antibody production
IL-8 Antibody (1A7C8), mAb, Mouse
Full Name IL-8 Antibody (1A7C8), mAb, Mouse
Abbreviated name-1
IL-8 Antibody, mAb, Mouse;
Documents
IL-8 Antibody (1A7C8), mAb, MouseDocument-MSDS: 12487_20100412223154.PDF (PDF)
IL-8 Antibody (1A7C8), mAb, MouseDocument-COA: 15684_20120314043949.PDF (PDF)
Figures
IL-8 Antibody (1A7C8), mAb, Mouse zoomIL-8 Antibody (1A7C8), mAb, Mouse
IL-8 Antibody (1A7C8), mAb, Mouse zoomIL-8 Antibody (1A7C8), mAb, Mouse
IL-8 Antibody (1A7C8), mAb, Mouse zoomIL-8 Antibody (1A7C8), mAb, Mouse
Citations
Description
IL-8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins is proinflammatory and primarily mediates the activation and migration of neutrophils from peripheral blood into tissue. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL-8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.
GenScript Human IL-8 Antibody (1A7C8), mAb, Mouse was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper, and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity, and native protein recognization.
Ig Subclass
IgG1, κ
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Human IL-8 Antibody (1A7C8), mAb, Mouse detects human IL-8 and does not cross-react with IL-1α, IL-1β, IL-4, IL-6, and IL-18.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Genetic immunization, amino acids sequence: MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KELKRAENS
Clone ID
1A7C8
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Predicted Band Size
36 KD
Observed Band Size
36 KD
Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 0.5-10 μg/ml
ELISA Detection: 0.05-0.2 μg/ml
Recommended antibody pairing for sandwich immunoassay:
Western blot: 0.1-1μg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Conjugation
Unconjugated
Species Reactivity
Human. Reactivity to other species is not tested yet.
Host Species
Mouse
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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