Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit (Gα(o)), which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. Gα(o) also belongs to the pertussis toxin sensitive G proteins. GenScript Gα(o) Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 50–100 of human Gα(o) (Swiss Prot: P09471).
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
GenScript Gα(o) Antibody, pAb, Rabbit detects endogenous levels of mouse and rat Gα(o). Predicted to react with human Gα(o) according to sequence homology. Positive Control: Mouse and rat brain
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 0.5-1 µg/ml Other Applications: User optimized
Western blot analysis of tissue lysates using Gα(o) Antibody, pAb, Rabbit (GenScript, A01616, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 40 KD Observed Size: 40 KD