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Gα(o) Antibody, pAb, Rabbit  |
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| Full Name |
Gα(o) Antibody, pAb, Rabbit
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Abbreviated name-1 |
GNAO1 Antibody, pAb, Rabbit; |
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Description |
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit (Gα(o)), which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. Gα(o) also belongs to the pertussis toxin sensitive G proteins.
GenScript Gα(o) Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 50–100 of human Gα(o) (Swiss Prot: P09471). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Gα(o) Antibody, pAb, Rabbit detects endogenous levels of mouse and rat Gα(o). Predicted to react with human Gα(o) according to sequence homology.
Positive Control: Mouse and rat brain
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide within residues 50–100 of human Gα(o) (Swiss Prot: P09471) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
40 KD |
Observed Band Size |
40 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: User optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Mouse and rat. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 11933_20100225223419.PDF (PDF)