Cat. No.
Name
Size   
Price
Selected
A01616-40
40 μg
$79 $47
Gα(o) Antibody, pAb, Rabbitcustom antibody production
Gα(o) Antibody, pAb, Rabbit
Full Name Gα(o) Antibody, pAb, Rabbit
Abbreviated name-1
GNAO1 Antibody, pAb, Rabbit;
Documents
Gα(o) Antibody, pAb, RabbitDocument-MSDS: 11933_20100225223419.PDF (PDF)
Figures
Gα(o) Antibody, pAb, Rabbit zoomGα(o) Antibody, pAb, Rabbit
Description
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit (Gα(o)), which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. Gα(o) also belongs to the pertussis toxin sensitive G proteins.
GenScript Gα(o) Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 50–100 of human Gα(o) (Swiss Prot: P09471).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Gα(o) Antibody, pAb, Rabbit detects endogenous levels of mouse and rat Gα(o). Predicted to react with human Gα(o) according to sequence homology.
Positive Control: Mouse and rat brain
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide within residues 50–100 of human Gα(o) (Swiss Prot: P09471)
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Predicted Band Size
40 KD
Observed Band Size
40 KD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: User optimized
Conjugation
Unconjugated
Species Reactivity
Mouse and rat. This product has not been tested with other species yet.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
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