|
DAZL antibody, pAb, Rabbit  |
|
|
| Full Name |
DAZL antibody, pAb, Rabbit
|
Abbreviated name-1 |
DAZL1 antibody, pAb, Rabbit; |
|
Description |
DAZL is one member of the DAZ family of RNA binding proteins. It plays a role in germ cell development. DAZL is located in the cytoplasm of adult germ cells. Mutations in DAZL have been implicated in severe spermatogenic failure and infertility in males. Expression of DAZL is found in fetal and adult testes and ovaries.
GenScript DAZL Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human DAZL (Swiss Prot: Q92904). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript DAZL Antibody, pAb, Rabbit detects endogenous levels of mouse and rat DAZL. Predicted to react with human DAZL according to sequence homology.
Positive Control: Mouse brain and rat brain
|
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from N-terminal of human DAZL (Swiss Prot: Q92904) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
33 KD |
Observed Band Size |
33 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: User optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Mouse and rat. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
|
 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
|
Document-MSDS: 11940_20100226001241.PDF (PDF)