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THE^TM HA Tag Antibody [FITC], mAb, Mouse

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THE^TM HA Tag Antibody [FITC], mAb, Mouse

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MSDS: 20100308205223 (PDF)
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Description

GenScript THE^TM HA Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE^TM HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of HA fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Ig Subclass

IgG1, k

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript THE^TM HA Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal HA tagged fusion proteins.

Concentration

1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide

Clone

5E11D8

Immunogen

A synthetic peptide YPYDVPDYA coupled to KLH

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized antibody with 100 μl of deionized water to the final concentration of 1 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/ml
Flow cytometry: 0.5-2 µg for 1 x 10⁶ cells
Other applications: User-optimized

Conjugation

Fluorescein isothiocyanate (FITC)

Host Species

Mouse

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)


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THETM HA Tag Antibody [FITC], mAb, Mouse

THE^TM HA Tag Antibody [FITC], mAb, Mouse