Neuron specific β-Tubulin III Antibody, pAb, Rabbit
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Neuron specific β-Tubulin III Antibody, pAb, Rabbit  |
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| Full Name |
Neuron specific β-Tubulin III Antibody, pAb, Rabbit
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Abbreviated name-1 |
Tubb3 Antibody, pAb, Rabbit; beta III Tubulin Antibody , pAb, Rabbit |
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Description |
Neuron specific β-Tubulin III, also known as tubulin beta-4, belongs to the tubulin family. It is the major constituent of microtubules and forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one binding is an exchangeable site of the beta chain and the other is at a non exchangeable site of the alpha chain. Neuron specific β-Tubulin III is regarded as a neuron-specific marker. The expression of neuron specific β-Tubulin III has been implicated in signal neuronal commitment in primitive neuroepithelium. In addition, increased expression of neuron specific β-Tubulin III occurs during cancer formation which leads to the development of anti-tubulin drugs.
GenScript Neuron Specific β-Tubulin III Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human neuron specific β-Tubulin III (Swiss Prot: Q13509). This antibody label neuronal cell bodies, dendrites, axons, and axonal terminations and is used identify newly committed neurons. In addition, it serves an excellent loading control when normalizing data specifically for neuronal expression.
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Neuron Specific β-Tubulin III Antibody, pAb, Rabbit detects endogenous levels of human, mouse, and rat neuron specific β-Tubulin III and does not cross-react with other beta tubulin isotypes.
Positive Control: Mouse brain, rat brain, SY-SH5H and U251. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from C-terminal of human neuron specific β-Tubulin III (Swiss Prot: Q13509) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
50 KD |
Observed Band Size |
50 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, and rat |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 12312_20100322235008.PDF (PDF)