Rho-tag Antibody, pAb, Rabbit
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Rho-tag Antibody, pAb, Rabbit  |
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| Full Name |
Rho-tag Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rho tag Antibody ; Rhodopsin Antibody |
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Description |
Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems.
The Rho-tag is an epitope tag consisting of a twenty-residue peptide, MNGTEGPNFYVPFSNKTGVV, which is derived from the first 20aa of bovine rhodopsin. Rho-tag can be used to boost expression levels of some eukaryotic membrane protein.
GenScript Rho-tag Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic Rho-tag epitope peptide.
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rho-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing Rho epitope tags. The antibody also recognizes mouse endogenous rhodopsin proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Developed in rabbit using a KLH-coupled synthetic Rho-tag epitope peptide |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
65 KD |
Observed Band Size |
65 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: User optimized
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Conjugation |
Unconjugated |
Host Species |
Rabbit |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 12346_20100326055706.PDF (PDF)
