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Nano-tag Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01629-200	Nano-tag Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01629-40	Nano-tag Antibody, pAb, Rabbit	40 μg	$50

Full Name

Nano-tag Antibody, pAb, Rabbit

Abbreviated name-1

Nano-Tag Antibody; Nano-tag9 Antibody; Nano-tag15 Antibody;

Documents
Document-MSDS: 12368_20100330235508.PDF (PDF)
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Reference

Description

Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems. The Nano-tag is a new streptavidin-binding peptide for both the purification and the detection of Nano-tagged proteins. This peptide possesses nanomolar-affinity for streptavidin and therefore is termed Nano-tag. The nano-tags have two types, Nano-tag15 (MDVEAWLGARVPLVET) and Nano-tag9 (MDVEAWLGAR), which bind to streptavidin with dissociation constants of 4 nM and 17 nM, respectively.
GenScript Nano-tag Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic Nano-tag epitope peptide.This antibody specifically reacts with fusion proteins containing Nano epitope tag. The antibody recognizes both Nano-tag9 and Nano-tag15 fusion protein.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic Nano-tag epitope peptide

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user optimized

Conjugation

Unconjugated

Host Species

Rabbit

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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