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THE^TM DYKDDDDK Tag Antibody [FITC], mAb, Mouse

Full Name

Abbreviated name-1

THE^TM Anti-DYKDDDDK [FITC] Monoclonal Antibody (Mouse)

Documents
MSDS: 20100308210619 (PDF)
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Description

GenScript THE^TM DYKDDDDK Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE^TM DYKDDDDK Tag Antibody, mAb, Mouse (A01428) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Ig Subclass

IgG2b, k

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript THE^TM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide

Clone

5A8E5

Immunogen

A synthetic peptide DYKDDDDK coupled to KLH

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/ml
Flow cytometry: 0.5-2 µg for 1 x 10⁶ cells
Other applications: User-optimized

Conjugation

Fluorescein isothiocyanate (FITC)

Host Species

Mouse

Technical Manual

ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)

Related Products

Cat. No.	Name	Size	Price
A00187	THE^TM DYKDDDDK Tag Antibody, mAb, Mouse	100 ug	$139.00
		40 ug	$68.00
		200 ug	$256.00
A01428	THE^TM DYKDDDDK Tag Antibody [HRP], mAb, Mouse	100 ug	$149.00
A01429	THE^TM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse	100 ug	$149.00
A00170	DYKDDDDK-tag Antibody, pAb, Rabbit	40 ug	$50.00
A00170	DYKDDDDK-tag Antibody, pAb, Rabbit	200 ug	$225.00


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THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse

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THE^TM DYKDDDDK Tag Antibody [FITC], mAb, Mouse