Catalog Products » Antibodies » antibody : alpha 1 Glycine Receptor Antibody, pAb, Rabbit
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A01636-40
40 μg
$79 $47
alpha 1 Glycine Receptor Antibody, pAb, Rabbitcustom antibody production
alpha 1 Glycine Receptor Antibody, pAb, Rabbit
Full Name alpha 1 Glycine Receptor Antibody, pAb, Rabbit
Abbreviated name-1
GLRA1 Antibody, pAb, Rabbit;
Documents
alpha 1 Glycine Receptor Antibody, pAb, RabbitDocument-MSDS: 12354_20100330031239.PDF (PDF)
Figures
alpha 1 Glycine Receptor Antibody, pAb, Rabbit zoomalpha 1 Glycine Receptor Antibody, pAb, Rabbit
Citations
Description
The glycine receptor is a neurotransmitter-gated ion channel. Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization. The alpha-1 subunit of the glycine receptor is a ligand-gated chloride channel. This inhibitory glycine receptor mediates postsynaptic inhibition in the spinal cord and other regions of the central nervous system. It is a pentameric receptor composed of alpha and beta subunits. Defects in alpha 1 Glycine Receptor are implicated in the pathophysiology of startle disease (STHE).
GenScript alpha 1 Glycine Receptor Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human alpha 1 Glycine Receptor (Swiss Prot: P23415).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript alpha 1 Glycine Receptor Antibody, pAb, Rabbit detects endogenous levels of mouse alpha 1 Glycine Receptor. It is predicted to react with human alpha 1 Glycine Receptor according to sequence homology.
Positive Control: Mouse brain
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
KLH-coupled synthetic peptide from N-terminal of human alpha 1 Glycine Receptor (Swiss Prot: P23415)
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Predicted Band Size
52 KD
Observed Band Size
48 KD
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/ml
Other Applications: user optimized
Conjugation
Unconjugated
Species Reactivity
Mouse. Reactivity to other species is not tested yet.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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