GenScript Share
Cat. No.
Name
Size   
Price
Selected
A01641-40
40 μg
$79 $47
A01641-200
200 μg(5x40μg)
$275
SNAI 1/2 Antibody, pAb, Rabbitcustom antibody production
SNAI 1/2 Antibody, pAb, Rabbit
Full Name SNAI 1/2 Antibody, pAb, Rabbit
Abbreviated name-1
SNAIL Antibody, pAb, Rabbit; SNAI2 Antibody, pAb, Rabbit
Documents
SNAI 1/2 Antibody, pAb, RabbitDocument-MSDS: 12699_20100430013746.PDF (PDF)
Figures
SNAI 1/2 Antibody, pAb, Rabbit zoomSNAI 1/2 Antibody, pAb, Rabbit
Citations
Description
SNAl protein family is a transcriptional repressor which contains SNAl1 and SNAI2. SNAl protein binds to the E-cadherin promoter region to repress transcription during development. The binding of SNAI protein to integrin promoter sequences represses integrin expression and results in reduced cell adhesion. The tumor suppressor protein p53 induces SNAL expression in γ-irradiated cells. Deletion mutations in the corresponding SNAI gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism.
GenScript SNAI 1/2 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human SNAl1 (Swiss Prot: O95863) and human SNAI2 (Swiss Prot: O43623).
Ig Subclass
IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript SNAI 1/2 Antibody, pAb, Rabbit detects endogenous levels of human SNAI 1/2. Predicted to react with mouse and rat SNAI2 according to sequence homology.
Positive Control: MCF-7, HEK293
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Immunogen
KLH-coupled synthetic peptide from C-terminal of human SNAl1 (Swiss Prot: O95863) and human SNAI2 (Swiss Prot: O43623)
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml.
Other Applications: User optimized.
Conjugation
Unconjugated
Species Reactivity
Human. Reactivity to other species is not tested yet.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.