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OLLAS-tag Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01658-200	OLLAS-tag Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01658-40	OLLAS-tag Antibody, pAb, Rabbit	40 μg	$50

Full Name

OLLAS-tag Antibody, pAb, Rabbit

Abbreviated name-1

OLLAS-tag Antibody;

Documents
Document-MSDS: 13554_20101226202535.PDF (PDF)
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Reference

Description

Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems. The OLLAS (SGFANELGPRLMGKC) epitope tag is a newly defined tag providing superior functionality on recombinant and fusion proteins without interfering with the biological activity of the tagged protein. GenScript OLLAS-tag Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic OLLAS-tag epitope peptide.

Ig Subclass

IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript OLLAS-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing OLLAS epitope tag.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic OLLAS-tag epitope peptide.

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1-2 µg/ml
Other Applications: user optimized

Conjugation

Unconjugated

Host Species

Rabbit

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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